Biocompatibility of a new pulp capping cement

Claudio Poggio; Matteo Ceci; Riccardo Beltrami; Alberto Dagna; Marco Colombo; Marco Chiesa

Biocompatibility of a new pulp capping cement

Ann Stomatol (Roma). 2014 Jun 18;5(2):69-76. eCollection 2014 Apr.

Authors

Claudio Poggio¹, Matteo Ceci¹, Riccardo Beltrami², Alberto Dagna¹, Marco Colombo¹, Marco Chiesa¹

Affiliations

¹ Department of Clinical, Surgical, Diagnostic and Pediatric Sciences, Section of Dentistry, University of Pavia, Italy.
² Department of Brain and Behavioural Sciences, University of Pavia, Italy.

PMID: 25002921
PMCID: PMC4071365

Abstract

Aim: THE AIM OF THE PRESENT STUDY WAS TO EVALUATE THE BIOCOMPATIBILITY OF A NEW PULP CAPPING MATERIAL (BIODENTINE, SEPTODONT) COMPARED WITH REFERENCE PULP CAPPING MATERIALS: Dycal (Dentsply), ProRoot MTA (Dentsply) and MTA-Angelus (Angelus) by using murine odontoblast cell line and Alamar blue and MTT cytotoxicity tests.

Methods: The citocompatibility of murine odontoblasts cells (MDPC-23) were evaluated at different times using a 24 Transwell culture plate by Alamar blue test and MTT assay.

Results: The results were significantly different among the pulp capping materials tested. Biocompatibility was significant different among materials with different composition.

Conclusions: Biodentine and MTA-based products show lower cytotoxicity varying from calcium hydroxide-based material which present higher citotoxicity.

Keywords: MTT test; biocompatibility; murine odontoblast; pulp capping materials.