Development of a liquid chromatographic method for the quantification of paromomycin. Application to in vitro release and ex vivo permeation studies

Spectrochim Acta A Mol Biomol Spectrosc. 2014 Dec 10:133:657-62. doi: 10.1016/j.saa.2014.06.017. Epub 2014 Jun 14.

Abstract

We have developed a reversed phase high performance liquid chromatography pulsed amperometric detection (RPHPLC-PAD) method for the determination of paromomycin. It is sensitive, repeatable, and selective without the pretreatment step. Trifluoroacetic acid-water was utilized as the eluent and detected by PAD under NaOH alkaline conditions. The paromomycin detection limit (S/N=3.3) was 2μgmL(-1) and the quantification limit (S/N=10) was 6μgmL(-1). Coefficients of linear regression were higher than 0.99 for concentrations between 6.25 and 200μgmL(-1). The intra and inter-day precision (RSD) was less than 6.5%. The average recoveries were 97.53-102.01%. The proposed HPLC-PAD method presented advantageous performance characteristics and it can be considered suitable for the evaluation of paromomycin loaded nanogel formulation in ex vivo permeation and in vitro release studies.

Keywords: Cutaneous leishmaniasis; Paromomycin; Pulsed amperometric detection; RPHPLC.

Publication types

  • Research Support, Non-U.S. Gov't
  • Validation Study

MeSH terms

  • Amebicides / analysis*
  • Amebicides / pharmacokinetics
  • Anti-Bacterial Agents / analysis*
  • Anti-Bacterial Agents / pharmacokinetics
  • Chromatography, Reverse-Phase / methods*
  • Electrochemical Techniques / methods
  • Humans
  • Limit of Detection
  • Paromomycin / analysis*
  • Paromomycin / pharmacokinetics
  • Skin / metabolism
  • Skin Absorption

Substances

  • Amebicides
  • Anti-Bacterial Agents
  • Paromomycin