Monitoring the efficacy of ADP inhibitor treatment in patients with acute STEMI post-PCI by VASP-P flow cytometry assay

Clin Appl Thromb Hemost. 2015 May;21(4):334-8. doi: 10.1177/1076029614540036. Epub 2014 Jul 2.

Abstract

Introduction: Dual antiplatelet treatment (DAPT) with clopidogrel and aspirin represents common approach in prevention of thromboembolic events in patients with acute coronary syndrome undergoing percutaneous coronary intervention (PCI). The drawback of clopidogrel treatment is large interindividual variability in response.

Aim: Our article aims to suggesting the most convenient method in monitoring the DAPT of post-PCI patients.

Methods: We analyzed the on-treatment platelet reactivity by light transmission aggregometry and vasodilator-stimulated phosphoprotein (VASP) flow cytometric assay. Samples were obtained in 3 intervals: first prior to PCI, then 1, and 30 days after PCI.

Results: Based on VASP-platelet reactivity index (PRI), we observed 100% response rate in prasugrel-treated patients and 62% to 73 % in the clopidogrel group. Overall, only 2 (7%) patients with the VASP-PRI value in therapeutic range had major adverse cardiovascular events.

Conclusion: Our results hint VASP-phosphorylation assay as a relevant method for guiding and tailoring DAPT.

Keywords: ADP inhibitor therapy; ST-segment elevation myocardial infarction; VASP-P; light transmission aggregometry; platelet reactivity index.

Publication types

  • Clinical Trial
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Acute Coronary Syndrome / blood
  • Acute Coronary Syndrome / drug therapy
  • Adenosine Diphosphate
  • Aged
  • Aged, 80 and over
  • Aspirin* / administration & dosage
  • Aspirin* / pharmacokinetics
  • Blood Platelets / metabolism
  • Cell Adhesion Molecules / blood*
  • Clopidogrel
  • Female
  • Flow Cytometry*
  • Follow-Up Studies
  • Humans
  • Male
  • Microfilament Proteins / blood*
  • Middle Aged
  • Myocardial Infarction* / blood
  • Myocardial Infarction* / drug therapy
  • Percutaneous Coronary Intervention*
  • Phosphoproteins / blood*
  • Platelet Activation / drug effects
  • Platelet Aggregation Inhibitors / administration & dosage*
  • Platelet Aggregation Inhibitors / pharmacokinetics
  • Prospective Studies
  • Ticlopidine / administration & dosage
  • Ticlopidine / analogs & derivatives*
  • Ticlopidine / pharmacokinetics

Substances

  • Cell Adhesion Molecules
  • Microfilament Proteins
  • Phosphoproteins
  • Platelet Aggregation Inhibitors
  • vasodilator-stimulated phosphoprotein
  • Adenosine Diphosphate
  • Clopidogrel
  • Ticlopidine
  • Aspirin