Abstract
The survival of T cells at different stages of development is dependent on extrinsic signals. IL-7 is necessary for the development of memory T cells. IL-7 could induce and maintain the differentiation, survival, and proliferation of CD4(+) memory T cells, and the roles of IL-2 and IL-15 in the generation of CD4(+) memory T cells were still unclear. A CD4(+) memory T cells in vitro generated system by adding IL-7. The phenotype of CD4(+) memory T cells was identified by FACS. The cells proliferation was analyzed by CFSE staining. The involved signal pathways were analyzed by Western blot. We found that IL-2, not IL-15, could inhibit CD4(+) memory T cells generation. Western blot showed that IL-7 up-regulated the P-STAT5A expression and down-regulated Bax expression, IL-2 reduced the effect of IL-7. Besides, IL-2-combined IL-7 up-regulated the P-AKT and Foxo3a expression a little. In conclusion, our data revealed the inhibitory role of IL-2 in CD4(+) memory T cells generation and indicated that PI3K/AKT signal pathway was involved.
Publication types
-
Research Support, Non-U.S. Gov't
MeSH terms
-
Animals
-
Bone Marrow Cells / cytology
-
CD4-Positive T-Lymphocytes / cytology*
-
CD4-Positive T-Lymphocytes / immunology
-
CD4-Positive T-Lymphocytes / metabolism
-
Dendritic Cells / cytology
-
Dendritic Cells / drug effects*
-
Dendritic Cells / immunology
-
Down-Regulation / drug effects
-
Forkhead Box Protein O3
-
Forkhead Transcription Factors / metabolism
-
Interleukin-15 / pharmacology
-
Interleukin-2 / pharmacology*
-
Interleukin-7 / pharmacology
-
Mice
-
Mice, Inbred C57BL
-
Mice, Transgenic
-
Ovalbumin / pharmacology
-
Phenotype
-
Phosphatidylinositol 3-Kinases / metabolism
-
Proto-Oncogene Proteins c-akt / metabolism
-
STAT5 Transcription Factor / metabolism
-
Signal Transduction / drug effects
-
Up-Regulation / drug effects
-
bcl-2-Associated X Protein / metabolism
Substances
-
Forkhead Box Protein O3
-
Forkhead Transcription Factors
-
FoxO3 protein, mouse
-
Interleukin-15
-
Interleukin-2
-
Interleukin-7
-
STAT5 Transcription Factor
-
Stat5a protein, mouse
-
bcl-2-Associated X Protein
-
Ovalbumin
-
Phosphatidylinositol 3-Kinases
-
Proto-Oncogene Proteins c-akt