Aurora kinase A is not involved in CPEB1 phosphorylation and cyclin B1 mRNA polyadenylation during meiotic maturation of porcine oocytes

PLoS One. 2014 Jul 1;9(7):e101222. doi: 10.1371/journal.pone.0101222. eCollection 2014.

Abstract

Regulation of mRNA translation by cytoplasmic polyadenylation is known to be important for oocyte maturation and further development. This process is generally controlled by phosphorylation of cytoplasmic polyadenylation element binding protein 1 (CPEB1). The aim of this study is to determine the role of Aurora kinase A in CPEB1 phosphorylation and the consequent CPEB1-dependent polyadenylation of maternal mRNAs during mammalian oocyte meiosis. For this purpose, we specifically inhibited Aurora kinase A with MLN8237 during meiotic maturation of porcine oocytes. Using poly(A)-test PCR method, we monitored the effect of Aurora kinase A inhibition on poly(A)-tail extension of long and short cyclin B1 encoding mRNAs as markers of CPEB1-dependent cytoplasmic polyadenylation. Our results show that inhibition of Aurora kinase A activity impairs neither cyclin B1 mRNA polyadenylation nor its translation and that Aurora kinase A is unlikely to be involved in CPEB1 activating phosphorylation.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Aurora Kinase A / metabolism*
  • Cyclin B1 / genetics*
  • Female
  • Meiosis*
  • Oocytes / enzymology
  • Oocytes / metabolism*
  • Phosphorylation
  • Polyadenylation
  • RNA, Messenger / metabolism*
  • Sus scrofa / metabolism
  • mRNA Cleavage and Polyadenylation Factors / chemistry
  • mRNA Cleavage and Polyadenylation Factors / metabolism*

Substances

  • Cyclin B1
  • RNA, Messenger
  • mRNA Cleavage and Polyadenylation Factors
  • Aurora Kinase A

Grants and funding

This work was supported by grant from the Czech Science Foundation P502/10/0944 (http://www.gacr.cz/) and by Institutional Research Concept 67985904 (IAPG AS CR, v.v.i., http://www.iapg.cas.cz/), AS was also supported by grant from the Czech Science Foundation 13- 12291S and MK was also supported by grant from the Czech Science Foundation P502/12/2201. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.