Abstract
The molecular epidemiology of 66 NDM-producing isolates from 2 Pakistani hospitals was investigated, with their genetic relatedness determined using repetitive sequence-based PCR (Rep-PCR). PCR-based replicon typing and screening for antibiotic resistance genes encoding carbapenemases, other β-lactamases, and 16S methylases were also performed. Rep-PCR suggested a clonal spread of Enterobacter cloacae and Escherichia coli. A number of plasmid replicon types were identified, with the incompatibility A/C group (IncA/C) being the most common (78%). 16S methylase-encoding genes were coharbored in 81% of NDM-producing Enterobacteriaceae.
Copyright © 2014, American Society for Microbiology. All Rights Reserved.
MeSH terms
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Acinetobacter baumannii / genetics*
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Acinetobacter baumannii / isolation & purification
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Acinetobacter baumannii / metabolism
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Amdinocillin / pharmacology
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Anti-Bacterial Agents / pharmacology
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Bacterial Proteins / genetics*
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Carbapenems / pharmacology
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Cross Infection / epidemiology
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DNA, Bacterial / genetics
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Doripenem
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Enterobacteriaceae / genetics*
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Enterobacteriaceae / isolation & purification
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Enterobacteriaceae / metabolism
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Fosfomycin / pharmacology
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Humans
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Meropenem
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Methyltransferases / genetics*
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Microbial Sensitivity Tests
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Molecular Epidemiology
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Multilocus Sequence Typing
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Pakistan
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Thienamycins / pharmacology
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beta-Lactamases / biosynthesis
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beta-Lactamases / genetics*
Substances
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Anti-Bacterial Agents
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Bacterial Proteins
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Carbapenems
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DNA, Bacterial
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Thienamycins
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Fosfomycin
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Doripenem
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Methyltransferases
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beta-Lactamases
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beta-lactamase NDM-1
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carbapenemase
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Meropenem
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Amdinocillin