Protein pathway analysis in Clinical Proteomics using protein microarrays

David H Geho; Virginia Espina; Julia Wulfkuhle; Emanuel F Petricoin; Lance A Liotta

doi:10.1016/j.ddtec.2005.11.008

Protein pathway analysis in Clinical Proteomics using protein microarrays

Drug Discov Today Technol. 2005 Winter;2(4):353-9. doi: 10.1016/j.ddtec.2005.11.008.

Authors

David H Geho¹, Virginia Espina², Julia Wulfkuhle², Emanuel F Petricoin², Lance A Liotta²

Affiliations

¹ The Center for Applied Proteomics and Molecular Medicine, George Mason University, 10900 University Blvd MS 4E3, Discovery Hall Room 182, Manassas, VA 20110, USA. Electronic address:dgeho@gmu.edu.
² The Center for Applied Proteomics and Molecular Medicine, George Mason University, 10900 University Blvd MS 4E3, Discovery Hall Room 182, Manassas, VA 20110, USA.

PMID: 24982012
DOI: 10.1016/j.ddtec.2005.11.008

Abstract

Molecular diagnostics research within the field of cancer is increasingly focused on detecting low-abundance protein endpoints that can be used to define a patient's disease more completely. Protein microarrays represent an important Clinical Proteomics tool for directly measuring protein endpoints in samples extracted from patient tissues. By combining laser capture microdissection, arraying devices, validated isoform-specific antibodies and advanced reporter technology tools, Clinical Proteomics laboratories are currently generating molecular portraits of cancer cells harvested from patient biopsies.: