High throughput, nanoliter quantitative PCR

Colin Brenan; Tom Morrison

doi:10.1016/j.ddtec.2005.08.017

High throughput, nanoliter quantitative PCR

Drug Discov Today Technol. 2005 Autumn;2(3):247-53. doi: 10.1016/j.ddtec.2005.08.017.

Authors

Colin Brenan¹, Tom Morrison²

Affiliations

¹ BioTrove Inc., 12 Gill Street, Suite 4000, Woburn, MA 01810, USA. Electronic address:cbrenan@biotrove.com.
² BioTrove Inc., 12 Gill Street, Suite 4000, Woburn, MA 01810, USA.

PMID: 24981943
DOI: 10.1016/j.ddtec.2005.08.017

Abstract

The recent completion of the human genome sequence has increased the need for high throughput quantitative transcription analysis. Quantitative PCR is an alternative to microarrays for accurate and precise expression analysis with single transcript copy sensitivity. A review of current research in miniaturized, high throughput qPCR suggests this technique will soon be a viable option to hybridization microarrays for large-scale genetic analyses.: