Genome sequence of Ensifer sp. TW10; a Tephrosia wallichii (Biyani) microsymbiont native to the Indian Thar Desert

Nisha Tak; Hukam S Gehlot; Muskan Kaushik; Sunil Choudhary; Ravi Tiwari; Rui Tian; Yvette Hill; Lambert Bräu; Lynne Goodwin; James Han; Konstantinos Liolios; Marcel Huntemann; Krishna Palaniappan; Amrita Pati; Konstantinos Mavromatis; Natalia Ivanova; Victor Markowitz; Tanja Woyke; Nikos Kyrpides; Wayne Reeve

doi:10.4056/sigs.4598281

Genome sequence of Ensifer sp. TW10; a Tephrosia wallichii (Biyani) microsymbiont native to the Indian Thar Desert

Stand Genomic Sci. 2013 Dec 15;9(2):304-14. doi: 10.4056/sigs.4598281. eCollection 2013 Dec 20.

Authors

Nisha Tak¹, Hukam S Gehlot¹, Muskan Kaushik¹, Sunil Choudhary¹, Ravi Tiwari², Rui Tian², Yvette Hill², Lambert Bräu³, Lynne Goodwin⁴, James Han⁵, Konstantinos Liolios⁵, Marcel Huntemann⁵, Krishna Palaniappan⁶, Amrita Pati⁵, Konstantinos Mavromatis⁵, Natalia Ivanova⁵, Victor Markowitz⁶, Tanja Woyke⁵, Nikos Kyrpides⁵, Wayne Reeve²

Affiliations

¹ BNF and Stress Biology Lab, Department of Botany, JNV University, Jodhpur, India.
² Centre for Rhizobium Studies, Murdoch University, Western Australia, Australia.
³ School of Life and Environmental Sciences, Faculty of Science, Engineering and Built Environment, Deakin University, Melbourne, Victoria, Australia.
⁴ Los Alamos National Laboratory, Bioscience Division, Los Alamos, New Mexico, USA.
⁵ DOE Joint Genome Institute, Walnut Creek, California, USA.
⁶ Biological Data Management and Technology Center, Lawrence Berkeley National Laboratory, Berkeley, California, USA.

Abstract

Ensifer sp. TW10 is a novel N2-fixing bacterium isolated from a root nodule of the perennial legume Tephrosia wallichii Graham (known locally as Biyani) found in the Great Indian (or Thar) desert, a large arid region in the northwestern part of the Indian subcontinent. Strain TW10 is a Gram-negative, rod shaped, aerobic, motile, non-spore forming, species of root nodule bacteria (RNB) that promiscuously nodulates legumes in Thar Desert alkaline soil. It is fast growing, acid-producing, and tolerates up to 2% NaCl and capable of growth at 40(o)C. In this report we describe for the first time the primary features of this Thar Desert soil saprophyte together with genome sequence information and annotation. The 6,802,256 bp genome has a GC content of 62% and is arranged into 57 scaffolds containing 6,470 protein-coding genes, 73 RNA genes and a single rRNA operon. This genome is one of 100 RNB genomes sequenced as part of the DOE Joint Genome Institute 2010 Genomic Encyclopedia for Bacteria and Archaea-Root Nodule Bacteria (GEBA-RNB) project.

Keywords: Alphaproteobacteria; nitrogen fixation; rhizobia; root-nodule bacteria.