Abstract
This study investigated the effect of arctigenin (Arc) on the cell activation, cytokines expression, proliferation, and cell-cycle distribution of mouse T lymphocytes. Mouse lymphocytes were prepared from lymph node and treated with Phorbol-12-myristate-13-acetate (PMA)/Ionimycin (Ion) and/or Arc. CD69, CD25, cytokines, proliferation and cell cycle were assayed by flow cytometry. The results showed that, at concentrations of less than 1.00 micromol x L(-1), Arc expressed non-obvious cell damage to cultured lymphocytes, however, it could significantly down-regulate the expression of CD69 and CD25, as well as TNF-alpha, IFN-gamma, IL-2, IL-4, IL-6 and IL-10 on PMA/Ion stimulated lymphocytes. At the same time, Arc could also inhibit the proliferation of PMA/Ion-activated lymphocytes and exhibited lymphocyte G 0/G1 phase cycle arrest. These results suggest that Arc possesses significant anti-inflammatory effects that may be mediated through the regulation of cell activation, cytokines expression and cell proliferation.
MeSH terms
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Animals
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Anti-Inflammatory Agents / isolation & purification
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Anti-Inflammatory Agents / pharmacology*
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Antigens, CD / metabolism
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Antigens, Differentiation, T-Lymphocyte / metabolism
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Arctium / chemistry
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Cell Cycle Checkpoints / drug effects
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Cell Proliferation / drug effects
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Cytokines / metabolism*
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Female
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Furans / isolation & purification
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Furans / pharmacology*
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Interferon-gamma / metabolism
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Interleukin-10 / metabolism
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Interleukin-2 / metabolism
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Interleukin-2 Receptor alpha Subunit / metabolism
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Interleukin-4 / metabolism
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Interleukin-6 / metabolism
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Ionomycin / pharmacology
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Lectins, C-Type / metabolism
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Lignans / isolation & purification
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Lignans / pharmacology*
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Lymphocyte Activation / drug effects*
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Mice
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Mice, Inbred BALB C
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Plants, Medicinal / chemistry
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T-Lymphocytes / cytology
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T-Lymphocytes / drug effects*
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T-Lymphocytes / immunology
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Tetradecanoylphorbol Acetate / pharmacology
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Tumor Necrosis Factor-alpha / metabolism
Substances
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Anti-Inflammatory Agents
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Antigens, CD
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Antigens, Differentiation, T-Lymphocyte
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CD69 antigen
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Cytokines
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Furans
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Interleukin-2
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Interleukin-2 Receptor alpha Subunit
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Interleukin-6
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Lectins, C-Type
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Lignans
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Tumor Necrosis Factor-alpha
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Interleukin-10
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Interleukin-4
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Ionomycin
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Interferon-gamma
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Tetradecanoylphorbol Acetate
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arctigenin