High-frequency ultrasonic imaging of growth and development in manufactured engineered oral mucosal tissue surfaces

Frank Winterroth; Hiroko Kato; Shiuhyang Kuo; Stephen E Feinberg; Scott J Hollister; J Brian Fowlkes; Kyle W Hollman

doi:10.1016/j.ultrasmedbio.2014.03.007

High-frequency ultrasonic imaging of growth and development in manufactured engineered oral mucosal tissue surfaces

Ultrasound Med Biol. 2014 Sep;40(9):2244-51. doi: 10.1016/j.ultrasmedbio.2014.03.007. Epub 2014 Jun 23.

Authors

Frank Winterroth¹, Hiroko Kato², Shiuhyang Kuo³, Stephen E Feinberg⁴, Scott J Hollister⁵, J Brian Fowlkes⁶, Kyle W Hollman⁷

Affiliations

¹ Department of Biomedical Engineering, University of Michigan, Ann Arbor, Michigan, USA; Department of Oral and Maxillofacial Surgery, University of Michigan, Ann Arbor, Michigan, USA. Electronic address: Fwinterr@umich.edu.
² Department of Oral and Maxillofacial Surgery, University of Michigan, Ann Arbor, Michigan, USA; Department of Oral Anatomy, Course for Oral Life Science, Niigata University Postgraduate School of Medical and Dental Sciences, Niigata, Japan.
³ Department of Oral and Maxillofacial Surgery, University of Michigan, Ann Arbor, Michigan, USA.
⁴ Department of Biomedical Engineering, University of Michigan, Ann Arbor, Michigan, USA; Department of Oral and Maxillofacial Surgery, University of Michigan, Ann Arbor, Michigan, USA.
⁵ Department of Biomedical Engineering, University of Michigan, Ann Arbor, Michigan, USA; Department of Surgery, University of Michigan, Ann Arbor, Michigan, USA; Department of Mechanical Engineering, University of Michigan, Ann Arbor, Michigan, USA.
⁶ Department of Radiology, University of Michigan, Ann Arbor, Michigan, USA; Department of Biomedical Engineering, University of Michigan, Ann Arbor, Michigan, USA.
⁷ Department of Biomedical Engineering, University of Michigan, Ann Arbor, Michigan, USA; Sound Sight Research, Livonia, Michigan, USA.

Abstract

This study uses high-resolution ultrasound to examine the growth and development of engineered oral mucosal tissues manufactured under aseptic conditions. The specimens are a commercially available natural tissue scaffold, AlloDerm, and oral keratinocytes seeded onto AlloDerm to form an ex vivo-produced oral mucosal equivalent (EVPOME) suitable for intra-oral grafting. The seeded cells produce a keratinized protective upper layer that smooths out any remaining surface irregularities on the underlying AlloDerm. Two-dimensional acoustic imaging of unseeded AlloDerm and developing EVPOMEs was performed on each day of their growth and development, each tissue specimen being imaged under aseptic conditions (total time from seeding to maturation: 11 d). Ultrasonic monitoring offers us the ability to determine the constituents of the EVPOME that are responsible for changes in its mechanical behavior during the manufacturing process. Ultrasonic monitoring affords us an opportunity to non-invasively assess, in real time, tissue-engineered constructs before release for use in patient care.

Keywords: Acoustic elasticity microscopy; Cell spreading; Image analysis; Keratinocyte; Non-invasive assessment; Oral mucosa; Tissue engineering; Ultrasound.

Publication types

Research Support, N.I.H., Extramural

MeSH terms

Cells, Cultured
Collagen*
Humans
Mouth Mucosa / diagnostic imaging*
Mouth Mucosa / growth & development*
Tissue Engineering / methods*
Tissue Scaffolds*
Ultrasonography

Substances

Alloderm
Collagen

Abstract

Publication types

MeSH terms

Substances

Grants and funding