Bio-reduction of redox-sensitive albumin conjugates in FcRn-expressing cells

Lorine Brülisauer; Gina Valentino; Sakura Morinaga; Kübra Cam; Jens Thostrup Bukrinski; Marc A Gauthier; Jean-Christophe Leroux

doi:10.1002/anie.201404238

Bio-reduction of redox-sensitive albumin conjugates in FcRn-expressing cells

Angew Chem Int Ed Engl. 2014 Aug 4;53(32):8392-6. doi: 10.1002/anie.201404238. Epub 2014 Jun 24.

Authors

Lorine Brülisauer¹, Gina Valentino, Sakura Morinaga, Kübra Cam, Jens Thostrup Bukrinski, Marc A Gauthier, Jean-Christophe Leroux

Affiliation

¹ Swiss Federal Institute of Technology Zurich (ETHZ), Department of Chemistry and Applied Biosciences, Institute of Pharmaceutical Sciences, Vladimir-Prelog-Weg 1-5/10, 8093 Zurich (Switzerland).

PMID: 24962346
DOI: 10.1002/anie.201404238

Abstract

Disulfide-containing IgG-, Fc-, or albumin-based prodrugs that rely on FcRn-trafficking by endothelial cells for prolonged circulation in the body might be hampered by premature bio-reduction processes during FcRn-mediated recycling events. A detailed bio-reduction analysis of redox-sensitive albumin conjugates in two FcRn-expressing cell lines has been performed. The obtained results indicate that the FcRn-mediated recycling pathway is not (or is only poorly) bio-reducing.

Keywords: FcRn receptors; albumin; disulfide; drug delivery; reduction.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Albumins / chemistry*
Drug Delivery Systems
Histocompatibility Antigens Class I / genetics*
Humans
Oxidation-Reduction
Receptors, Fc / genetics*

Substances

Albumins
Histocompatibility Antigens Class I
Receptors, Fc
Fc receptor, neonatal