Cytopathic effect of Human cosavirus (HCoSV) on primary cell cultures of human embryonic lung MRC5

Dorra Rezig; Henda Touzi; Zina Meddeb; Henda Triki

doi:10.1016/j.jviromet.2014.06.011

Cytopathic effect of Human cosavirus (HCoSV) on primary cell cultures of human embryonic lung MRC5

J Virol Methods. 2014 Oct:207:12-5. doi: 10.1016/j.jviromet.2014.06.011. Epub 2014 Jun 21.

Authors

Dorra Rezig¹, Henda Touzi², Zina Meddeb², Henda Triki²

Affiliations

¹ Laboratory of Clinical Virology, WHO Regional Reference Laboratory on Poliomyelitis and Measles, Institut Pasteur de Tunis, Tunisia. Electronic address: dor_rare@yahoo.fr.
² Laboratory of Clinical Virology, WHO Regional Reference Laboratory on Poliomyelitis and Measles, Institut Pasteur de Tunis, Tunisia.

PMID: 24960630
DOI: 10.1016/j.jviromet.2014.06.011

Abstract

Human cosaviruses (HCoSVs) are newly discovered viruses in Picornaviridae family. Until now, most published studies reported HCoSV detection using molecular techniques and genetic characterization of the virus. Nevertheless, no laboratory has yet reported the replication of these viruses in cultured cell lines. In the present work, the propagation of HCoSV strains isolated from human fecal specimens in MRC5 cell line and their induced cytopathic effects (CPE) was studied. The first sign of virus growth was observed 24-48h after inoculation. The cells rounded up and clumped together rapidly; empty areas became visible and, on the third day of CPE, a remarkable decrease in live cells was observed. This represents the first report on in vitro model of HCoSV replication which opens up opportunities for future investigations of these new viruses.

Keywords: Cell culture; Cytopathic effect; Human cosavirus; MRC5; Picornaviridae.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Cell Line
Cytopathogenic Effect, Viral*
Humans
Picornaviridae / growth & development
Picornaviridae / pathogenicity
Picornaviridae / physiology*
Virus Cultivation