Capture and identification of the volatile components in crude and processed herbal medicines through on-line purge and trap technique coupled with GC × GC-TOF MS

Gang Cao; Zhiwei Xu; Xin Wu; Qingli Li; Xiaocheng Chen

doi:10.1080/14786419.2014.929129

Capture and identification of the volatile components in crude and processed herbal medicines through on-line purge and trap technique coupled with GC × GC-TOF MS

Nat Prod Res. 2014;28(19):1607-12. doi: 10.1080/14786419.2014.929129. Epub 2014 Jun 24.

Authors

Gang Cao¹, Zhiwei Xu, Xin Wu, Qingli Li, Xiaocheng Chen

Affiliation

¹ a Research Center of TCM Processing Technology, Zhejiang Chinese Medical University , Hangzhou , P.R. China.

PMID: 24960332
DOI: 10.1080/14786419.2014.929129

Abstract

This work aimed to investigate the volatile components in crude and processed herbal medicines (HMs). Using Atractylodis Macrocephalae Rhizoma (AMR) as a model HM, the volatile components were captured through on-line purge and trap technique and identified by using comprehensive two-dimensional gas chromatography-time-of-flight mass spectrometry (GC × GC-TOF MS) system. A total of 224 and 171 volatile compounds were identified in crude and processed AMR samples, respectively. After frying with honey-bran, 52 compounds which were found in crude AMR samples disappeared in processed AMR samples, and 15 compounds were newly generated in processed AMR. The established method can be applied in different research areas such as HM and food processing.

Keywords: Atractylodis Macrocephalae Rhizoma; herbal medicines; on-line purge and trap; two-dimensional gas chromatography–time-of-flight mass spectrometry; volatile components.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Drugs, Chinese Herbal / chemistry*
Gas Chromatography-Mass Spectrometry
Herbal Medicine
Oils, Volatile / analysis*
Plants, Medicinal / chemistry*
Rhizome / chemistry

Substances

Drugs, Chinese Herbal
Oils, Volatile