Abstract
Five new prenylated benzoylphloroglucinol derivatives, garciesculentones A-E (1-5), a new xanthone, garciesculenxanthone A (6), and 15 known compounds were isolated from the petroleum ether extract and the EtOAc-soluble fraction of a 80% (v/v) EtOH extract of Garcinia esculenta. The structures of the new compounds were elucidated by 1D- and 2D-NMR spectroscopic analysis and mass spectrometry. Experimental and calculated ECD and a convenient modified Mosher's method were used to determine the absolute configurations. The cytotoxicity of these compounds were evaluated by MTT assay against three human cancer cell lines (HepG2, MCF-7, and MDA-MB-231) and against normal hepatic cells (HL-7702). In addition, these isolates were evaluated for their inhibitory effects on interferon-γ plus lipopolysaccharide-induced nitric oxide production in RAW264.7 cells.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Anti-Inflammatory Agents* / chemistry
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Anti-Inflammatory Agents* / isolation & purification
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Anti-Inflammatory Agents* / pharmacology
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Antineoplastic Agents, Phytogenic* / chemistry
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Antineoplastic Agents, Phytogenic* / isolation & purification
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Antineoplastic Agents, Phytogenic* / pharmacology
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Drug Screening Assays, Antitumor
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Drugs, Chinese Herbal* / chemistry
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Drugs, Chinese Herbal* / isolation & purification
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Drugs, Chinese Herbal* / pharmacology
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Female
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Garcinia / chemistry*
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Hep G2 Cells
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Humans
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Interferon-gamma / drug effects
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Lipopolysaccharides / pharmacology
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Mice
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Molecular Structure
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Nitric Oxide / biosynthesis
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Nuclear Magnetic Resonance, Biomolecular
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Phloroglucinol* / analogs & derivatives
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Phloroglucinol* / chemistry
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Phloroglucinol* / isolation & purification
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Phloroglucinol* / pharmacology
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Prenylation
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Xanthones* / chemistry
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Xanthones* / isolation & purification
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Xanthones* / pharmacology
Substances
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Anti-Inflammatory Agents
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Antineoplastic Agents, Phytogenic
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Drugs, Chinese Herbal
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Lipopolysaccharides
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Xanthones
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Nitric Oxide
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Interferon-gamma
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Phloroglucinol