Objective: To optimize the extraction conditions for triphyllin A in Pronephrium triphyllum.
Methods: The content of triphyllin A in the Pronephrium triphyllum was determined by HPLC. Concentration and volume of the alcohol,time and times of the extraction were assayed by orthogonal test to detect their influences on the extraction rate of triphyllin A in the Pronephrium triphyllum.
Results: Alcohol volume and extraction times had significant influence on the process (P < 0.05) while alcohol concentration and extraction time had no effect. The optimal extraction conditions were as follows:50 fold 60% alcohol, extraction for 3 times and 50 min for each time.
Conclusion: The extration rate of triphyllin A is higher,and the process can be used for the development and production of Pronephrium triphyllum.