Comparable constitutive expression and activity of cytochrome P450 between the lobes of the porcine liver

Martin Krøyer Rasmussen; Galia Zamaratskaia; Bo Ekstrand

doi:10.1016/j.tiv.2014.06.002

Comparable constitutive expression and activity of cytochrome P450 between the lobes of the porcine liver

Toxicol In Vitro. 2014 Oct;28(7):1190-5. doi: 10.1016/j.tiv.2014.06.002. Epub 2014 Jun 18.

Authors

Martin Krøyer Rasmussen¹, Galia Zamaratskaia², Bo Ekstrand³

Affiliations

¹ Department of Food Science, Aarhus University, Denmark. Electronic address: Martink.rasmussen@agrsci.dk.
² Department of Food Science, Swedish University of Agricultural Science, Uppsala, Sweden.
³ Department of Food Science, Aarhus University, Denmark.

PMID: 24952075
DOI: 10.1016/j.tiv.2014.06.002

Abstract

Due to limited availability of human liver tissue for the study of cytochrome P450 (CYP450), porcine liver tissue has been suggested as an alternative source to prepare microsomes and hepatocytes. The porcine liver is made by four different lobes. The present study investigated the expression and activity of specific CYP450 isoforms in the four lobes, with the purpose to examine if one lobe of the porcine liver resembles the human more than others. Samples from the four major lobes were taken from female pigs and mRNA expression and activity of CYP1A, 2A, 2C, 2D, 2E and 3A determined. The results showed no differences in specific mRNA expression and activity of any of the investigated CYP450 isoforms. In conclusion, the study shows that all parts of the porcine liver are equally useful as model tissue.

Keywords: Detoxification; Inter-individual; Intra-hepatic; Model system; PCA; Porcine liver.

Publication types

Comparative Study

MeSH terms

Animals
Cytochrome P-450 Enzyme System / genetics*
Cytochrome P-450 Enzyme System / metabolism*
Female
Liver / metabolism*
Microsomes, Liver / metabolism
RNA, Messenger / metabolism
Swine

Substances

RNA, Messenger
Cytochrome P-450 Enzyme System