Involvement of leukotriene B4 in dermatophyte-related itch in mice

Pharmacol Rep. 2014 Aug;66(4):699-703. doi: 10.1016/j.pharep.2014.01.003. Epub 2014 Apr 26.

Abstract

Background: Proteinase-activated receptor-2 (PAR2) is involved in dermatophyte-induced scratching and leukotriene B4 (LTB4) release from keratinocytes. We investigated whether PAR2-mediated LTB4 production is involved in dermatophyte-induced scratching.

Methods: Dermatophyte extract was injected intradermally and scratching was observed in mice. LTB4 was determined by enzyme immunoassay.

Results: Dermatophyte extract-induced scratching was inhibited by zileuton (5-lipoxygenase inhibitor), ONO-4057 (LTB4 antagonist), FSLLRY-NH2 (PAR2 antagonist), and anti-PAR2 antibody. Dermatophyte extract injection increased the cutaneous content of LTB4, which was inhibited by zileuton and FSLLRY-NH2.

Conclusion: These results suggest the involvement of LTB4 in dermatophyte-associated itch. LTB4 production might be due to PAR2 stimulation in the skin.

Keywords: 5-Lipoxygenase; Dermatophyte; Itch; Leukotriene B(4); Proteinase-activated receptor 2.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Antibodies, Monoclonal / administration & dosage
  • Antibodies, Monoclonal / therapeutic use
  • Arthrodermataceae / pathogenicity
  • Dermatomycoses / complications
  • Dermatomycoses / drug therapy
  • Dermatomycoses / metabolism*
  • Hydroxyurea / administration & dosage
  • Hydroxyurea / analogs & derivatives
  • Hydroxyurea / therapeutic use
  • Keratinocytes / metabolism
  • Leukotriene Antagonists / administration & dosage
  • Leukotriene Antagonists / therapeutic use
  • Leukotriene B4 / antagonists & inhibitors
  • Leukotriene B4 / metabolism*
  • Male
  • Mice, Inbred Strains
  • Oligopeptides / administration & dosage
  • Oligopeptides / therapeutic use
  • Pruritus / drug therapy
  • Pruritus / etiology
  • Pruritus / metabolism*
  • Receptor, PAR-2 / antagonists & inhibitors
  • Receptor, PAR-2 / immunology
  • Receptor, PAR-2 / metabolism

Substances

  • Antibodies, Monoclonal
  • H-Phe-Ser-Leu-Leu-Arg-Tyr-NH2
  • Leukotriene Antagonists
  • Oligopeptides
  • Receptor, PAR-2
  • Leukotriene B4
  • zileuton
  • Hydroxyurea