Portable pH-inspired electrochemical detection of DNA amplification

Chem Commun (Camb). 2014 Aug 7;50(61):8416-9. doi: 10.1039/c4cc03011g.

Abstract

A portable and label-free pH-mediated electrochemical method for the detection of DNA amplification is described. With protons released as readouts, DNA amplifications were detected in real-time or at the end-point.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Agrobacterium tumefaciens / enzymology
  • Amino Acid Oxidoreductases / genetics
  • DNA / analysis*
  • DNA / metabolism
  • Electrochemical Techniques*
  • Hydrogen-Ion Concentration
  • Nucleic Acid Amplification Techniques

Substances

  • DNA
  • Amino Acid Oxidoreductases
  • nopaline synthase