Cellular proteomics of total proteins in ovaries of domesticated and wild giant tiger shrimp (Penaeus monodon) were examined using GeLC-MS/MS. In total, 1638 proteins matched those previously deposited in databases and 1253 (76.50%) of these significantly matched known proteins. Several reproduction-related proteins (e.g. Cdc2, Cyclin B, Cdc25, 14-3-3, thymosin-β and Rac-GTPase activating protein 1) were identified. In addition, the full-length cDNA of P. monodon thymosin-β (PmTmsb; 1084 bp with an ORF of 387 bp and 128 deduced aa) and Rac-GTPase activating protein 1 (PmRacgap1; an ORF of 1881 bp and 626 deduced aa) were further characterized. PmTmsb was constitutively expressed in all tissues. In contrast, PmRacgap1 was more abundantly expressed in gonads than in several non-reproductive tissues (e.g. subcuticular epithelium, hepatopancreas, intestine, pleopods, stomach and thoracic ganglion). The expression levels of PmTmsb and PmRacgap1 in ovaries of wild adult broodstock were significantly greater than those in ovaries of juveniles (P<0.05). However, their expression levels did not vary significantly during ovarian development stages in intact broodstock. However, eyestalk ablation resulted in a significant reduction in PmTmsb expression at stages I and III ovaries (P<0.05), although it did not affect PmRacgap1 transcription significantly at these stages. On the other hand, use of polyclonal antibodies derived from recombinant PmTmsb and PmRacgap1 revealed that levels of both proteins decreased at the late stage (IV) of ovarian development. Our results suggested that PmTmsb and PmRacgap1 may act as negative effectors during ovarian development in P. monodon.
Keywords: Ovarian proteomics; Penaeus monodon; Racgap1; Real-time PCR; Thymosin-β.
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