Secretory vesicles deliver Cdc42p to sites of polarized growth in S. cerevisiae

Shubha A Dighe; Keith G Kozminski

doi:10.1371/journal.pone.0099494

Secretory vesicles deliver Cdc42p to sites of polarized growth in S. cerevisiae

PLoS One. 2014 Jun 19;9(6):e99494. doi: 10.1371/journal.pone.0099494. eCollection 2014.

Authors

Shubha A Dighe¹, Keith G Kozminski²

Affiliations

¹ Department of Biology, University of Virginia, Charlottesville, Virginia, United States of America.
² Department of Biology, University of Virginia, Charlottesville, Virginia, United States of America; Department of Cell Biology, University of Virginia, Charlottesville, Virginia, United States of America.

Abstract

The activation and localization of the Rho-family GTPase Cdc42p at one pole of a cell is necessary for maintaining an axis of polarized growth in many animal and fungal cells. How the asymmetric distribution of this key regulator of polarized morphogenesis is maintained is not fully understood, though divergent models have emerged from a congruence of multiple studies, including one that posits a role for polarized secretion. Here we show with S. cerevisiae that Cdc42p associates with secretory vesicles in vivo.

Publication types

Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

Cell Polarity
Endoplasmic Reticulum / metabolism*
Endoplasmic Reticulum / ultrastructure
Gene Expression
Microscopy, Electron
Phosphoproteins / genetics
Phosphoproteins / metabolism*
Protein Binding
Protein Transport
Saccharomyces cerevisiae / genetics
Saccharomyces cerevisiae / growth & development
Saccharomyces cerevisiae / metabolism*
Saccharomyces cerevisiae / ultrastructure
Secretory Vesicles / metabolism*
Secretory Vesicles / ultrastructure
cdc42 GTP-Binding Protein, Saccharomyces cerevisiae / genetics
cdc42 GTP-Binding Protein, Saccharomyces cerevisiae / metabolism*

Substances

Phosphoproteins
cdc42 GTP-Binding Protein, Saccharomyces cerevisiae

Grants and funding

Funds for this work were provided by the NSF (grant #0723342) and the University of Virginia. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.