Two missense mutations, E123Q and K151E, identified in the ERG11 allele of an azole-resistant isolate of Candida kefyr recovered from a stem cell transplant patient for acute myeloid leukemia

Célia Couzigou; Frédéric Gabriel; Nicolas Biteau; Valérie Fitton-Ouhabi; Thierry Noël; Isabelle Accoceberry

doi:10.1016/j.mmcr.2014.04.002

Two missense mutations, E123Q and K151E, identified in the ERG11 allele of an azole-resistant isolate of Candida kefyr recovered from a stem cell transplant patient for acute myeloid leukemia

Med Mycol Case Rep. 2014 May 9:5:12-5. doi: 10.1016/j.mmcr.2014.04.002. eCollection 2014 Jul.

Authors

Célia Couzigou¹, Frédéric Gabriel¹, Nicolas Biteau², Valérie Fitton-Ouhabi², Thierry Noël², Isabelle Accoceberry¹

Affiliations

¹ Université de Bordeaux, Microbiologie Fondamentale et Pathogénicité UMR 5234, F-33000 Bordeaux, France ; CNRS, Microbiologie Fondamentale et Pathogénicité, UMR 5234, F-33000 Bordeaux, France ; Centre Hospitalier Universitaire de Bordeaux, Laboratoire de Parasitologie-Mycologie, F-33000 Bordeaux, France.
² Université de Bordeaux, Microbiologie Fondamentale et Pathogénicité UMR 5234, F-33000 Bordeaux, France ; CNRS, Microbiologie Fondamentale et Pathogénicité, UMR 5234, F-33000 Bordeaux, France.

Abstract

We report on the first cloning and nucleotide sequencing of an ERG11 allele from a clinical isolate of Candida kefyr cross-resistant to azole antifungals. It was recovered from a stem cell transplant patient, in an oncohematology unit exhibiting unexpected high prevalence of C. kefyr. Two amino acid substitutions were identified: K151E, whose role in fluconazole resistance was already demonstrated in Candida albicans, and E123Q, a new substitution never described so far in azole-resistant Candida yeast.

Keywords: Azole resistance; Candida kefyr; ERG11; Kluyveromyces marxianus; Single nucleotide polymorphism.