Identification of tumor promoters by their inhibitory effect on intercellular transfer of lucifer yellow

Cell Biol Toxicol. 1989 Jan;5(1):77-89. doi: 10.1007/BF00141066.

Abstract

The effect of the tumor promoters 12-O-tetradecanoylphorbol-13-acetate (TPA), mezerein, teleocidin, anthralin, the Ca2+-ionophore A23187, butylated hydroxytoluene (BHT), dichlorodiphenyltrichloroethane (DDT) and phenobarbital (PB) on lucifer yellow transfer in cultures of SV-40-transformed Djungarian hamster fibroblasts was studied. TPA, mezerein, teleocidin, A23187, DDT and BHT exerted a strong inhibitory effect on cell-to-cell dye transfer. Anthralin uncoupled cells in 3 experiments out of 6. PB appeared to enhance lucifer yellow transfer. Sodium nitrite, a substance with unknown promoting activity, effectively uncoupled cells. All the promoters investigated had a reversible effect on the dye transfer. The value of the dye transfer method for promoter screening is discussed.

MeSH terms

  • Animals
  • Anthralin / analysis
  • Anthralin / toxicity
  • Butylated Hydroxytoluene / analysis
  • Butylated Hydroxytoluene / toxicity
  • Calcimycin / analysis
  • Calcimycin / toxicity
  • Carcinogens / analysis
  • Carcinogens / toxicity*
  • Cells, Cultured
  • Cricetinae
  • DDT / analysis
  • DDT / toxicity
  • Diterpenes*
  • Fluorescent Dyes
  • Intercellular Junctions / drug effects*
  • Isoquinolines*
  • Lyngbya Toxins / analysis
  • Lyngbya Toxins / toxicity
  • Phenobarbital / analysis
  • Phenobarbital / toxicity
  • Terpenes / analysis
  • Terpenes / toxicity
  • Tetradecanoylphorbol Acetate / analysis
  • Tetradecanoylphorbol Acetate / toxicity

Substances

  • Carcinogens
  • Diterpenes
  • Fluorescent Dyes
  • Isoquinolines
  • Lyngbya Toxins
  • Terpenes
  • Butylated Hydroxytoluene
  • teleocidins
  • mezerein
  • Calcimycin
  • lucifer yellow
  • DDT
  • Tetradecanoylphorbol Acetate
  • Anthralin
  • Phenobarbital