Abstract
The effect of the tumor promoters 12-O-tetradecanoylphorbol-13-acetate (TPA), mezerein, teleocidin, anthralin, the Ca2+-ionophore A23187, butylated hydroxytoluene (BHT), dichlorodiphenyltrichloroethane (DDT) and phenobarbital (PB) on lucifer yellow transfer in cultures of SV-40-transformed Djungarian hamster fibroblasts was studied. TPA, mezerein, teleocidin, A23187, DDT and BHT exerted a strong inhibitory effect on cell-to-cell dye transfer. Anthralin uncoupled cells in 3 experiments out of 6. PB appeared to enhance lucifer yellow transfer. Sodium nitrite, a substance with unknown promoting activity, effectively uncoupled cells. All the promoters investigated had a reversible effect on the dye transfer. The value of the dye transfer method for promoter screening is discussed.
MeSH terms
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Animals
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Anthralin / analysis
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Anthralin / toxicity
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Butylated Hydroxytoluene / analysis
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Butylated Hydroxytoluene / toxicity
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Calcimycin / analysis
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Calcimycin / toxicity
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Carcinogens / analysis
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Carcinogens / toxicity*
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Cells, Cultured
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Cricetinae
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DDT / analysis
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DDT / toxicity
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Diterpenes*
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Fluorescent Dyes
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Intercellular Junctions / drug effects*
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Isoquinolines*
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Lyngbya Toxins / analysis
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Lyngbya Toxins / toxicity
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Phenobarbital / analysis
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Phenobarbital / toxicity
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Terpenes / analysis
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Terpenes / toxicity
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Tetradecanoylphorbol Acetate / analysis
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Tetradecanoylphorbol Acetate / toxicity
Substances
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Carcinogens
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Diterpenes
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Fluorescent Dyes
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Isoquinolines
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Lyngbya Toxins
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Terpenes
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Butylated Hydroxytoluene
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teleocidins
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mezerein
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Calcimycin
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lucifer yellow
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DDT
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Tetradecanoylphorbol Acetate
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Anthralin
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Phenobarbital