From fluorescence polarization to Quenchbody: Recent progress in fluorescent reagentless biosensors based on antibody and other binding proteins

Hiroshi Ueda; Jinhua Dong

doi:10.1016/j.bbapap.2014.06.005

From fluorescence polarization to Quenchbody: Recent progress in fluorescent reagentless biosensors based on antibody and other binding proteins

Biochim Biophys Acta. 2014 Nov;1844(11):1951-1959. doi: 10.1016/j.bbapap.2014.06.005. Epub 2014 Jun 13.

Authors

Hiroshi Ueda¹, Jinhua Dong²

Affiliations

¹ Chemical Resources Laboratory, Tokyo Institute of Technology, 4259-R1-18, Nagatsuta-cho, Midori-ku, Yokohama, Kanagawa 226-8503 Japan. Electronic address: ueda@res.titech.ac.jp.
² Chemical Resources Laboratory, Tokyo Institute of Technology, 4259-R1-18, Nagatsuta-cho, Midori-ku, Yokohama, Kanagawa 226-8503 Japan.

PMID: 24931832
DOI: 10.1016/j.bbapap.2014.06.005

Abstract

Recently, antibody-based fluorescent biosensors are receiving considerable attention as a suitable biomolecule for diagnostics, namely, homogeneous immunoassay and also as an imaging probe. To date, several strategies for "reagentless biosensors" based on antibodies and natural and engineered binding proteins have been described. In this review, several approaches are introduced including a recently described fluorescent antibody-based biosensor Quenchbody, which works on the principle of fluorescence quenching of attached dye and its antigen-dependent release. The merits and possible demerits of each approach are discussed. This article is part of a Special Issue entitled: Recent advances in molecular engineering of antibody.

Keywords: DARPin; Fluorescence quenching; In vitro translation; Position-specific labeling; Q-body; Reagentless biosensor.

Publication types

Review