Nanopores formed by DNA origami: a review

Nicholas A W Bell; Ulrich F Keyser

doi:10.1016/j.febslet.2014.06.013

Nanopores formed by DNA origami: a review

FEBS Lett. 2014 Oct 1;588(19):3564-70. doi: 10.1016/j.febslet.2014.06.013. Epub 2014 Jun 10.

Authors

Nicholas A W Bell¹, Ulrich F Keyser²

Affiliations

¹ Cavendish Laboratory, Cambridge University, J J Thomson Avenue, Cambridge CB3 0HE, United Kingdom.
² Cavendish Laboratory, Cambridge University, J J Thomson Avenue, Cambridge CB3 0HE, United Kingdom. Electronic address: ufk20@cam.ac.uk.

PMID: 24928438
DOI: 10.1016/j.febslet.2014.06.013

Abstract

Nanopores have emerged over the past two decades to become an important technique in single molecule experimental physics and biomolecule sensing. Recently DNA nanotechnology, in particular DNA origami, has been used for the formation of nanopores in insulating materials. DNA origami is a very attractive technique for the formation of nanopores since it enables the construction of 3D shapes with precise control over geometry and surface functionality. DNA origami has been applied to nanopore research by forming hybrid architectures with solid state nanopores and by direct insertion into lipid bilayers. This review discusses recent experimental work in this area and provides an outlook for future avenues and challenges.

Keywords: DNA origami; Nanopores; Nanotechnology; Self-assembly; Single molecule.

Publication types

Research Support, Non-U.S. Gov't
Review

MeSH terms

DNA / chemistry*
Lipid Bilayers / chemistry
Nanopores*
Nanotechnology / methods*
Nucleic Acid Conformation*

Substances

Lipid Bilayers
DNA