Expression of claudin-3 in the esophagus and larynx of rat reflux model

Auris Nasus Larynx. 2014 Dec;41(6):539-42. doi: 10.1016/j.anl.2014.05.011. Epub 2014 Jun 11.

Abstract

Objective: The aim of this study was to investigate the association between laryngeal expression of claudin-3 and laryngopharyngeal reflux (LPR) in a rat reflux model.

Methods: Eight Wistar rats were divided into two groups. Four rats underwent total esophageal myectomy to induce reflux, and the remainder underwent a sham operation as a control. All animals were sacrificed 12 weeks after surgery to perform tissue histology and Western blot analysis.

Results: Lymphocyte infiltration increased significantly in the study group in both esophageal and laryngeal samples (P=0.001, 0.002, respectively). Both esophageal and laryngeal expressions of claudin-3 were significantly lower in the study group when compared with that in the control group (P=0.045, 0.037, respectively).

Conclusion: The results of this study suggest that a decrease in claudin-3 could be a sensitive indicator of reflux laryngitis in rats.

Keywords: Claudin-3; Gastroesophageal reflux disease; Laryngopharyngeal reflux.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Blotting, Western
  • Claudin-3 / metabolism*
  • Disease Models, Animal
  • Esophagitis / etiology
  • Esophagitis / metabolism*
  • Esophagitis / pathology
  • Esophagus / metabolism*
  • Esophagus / pathology
  • Gastroesophageal Reflux / complications
  • Gastroesophageal Reflux / metabolism
  • Gastroesophageal Reflux / pathology
  • Laryngitis / etiology
  • Laryngitis / metabolism*
  • Laryngitis / pathology
  • Laryngopharyngeal Reflux / complications
  • Laryngopharyngeal Reflux / metabolism*
  • Laryngopharyngeal Reflux / pathology
  • Larynx / metabolism*
  • Larynx / pathology
  • Rats
  • Rats, Wistar

Substances

  • Claudin-3
  • Cldn3 protein, rat