Mapping of the exchangeable and dispensable domains of the RNA 2-encoded 2A(HP) protein of arabis mosaic nepovirus

Virology. 2014 Jun:458-459:106-13. doi: 10.1016/j.virol.2014.04.025. Epub 2014 May 10.

Abstract

The N-terminal domains of the RNA 2-encoded 2A(HP) proteins of the arabis mosaic (ArMV) and grapevine fanleaf (GFLV) nepoviruses were shown to be highly variable and a hotspot for intra- and inter-species recombination events. Chimeric ArMV-NW clones in which the N-terminal domain of 2A(HP) or the entire 2A(HP) of GFLV isolates replaced the corresponding domains of ArMV retained their infectivity, showing that the 2A(HP) proteins of ArMV-NW and GFLV are exchangeable. ArMN-NW clones with deletions of the N-terminal, core, or C-terminal domains of the ArMV-NW 2A(HP) were infectious in Chenopodium quinoa although viral RNA (especially RNA 2) accumulated at reduced levels. In contrast, deletion of the entire 2A(HP) protein or of the C-terminal two thirds of the protein abolished infectivity of the ArMV-NW clones. These results suggest that multiple functional domains are distributed throughout the 2A(HP) protein and are essential for the accumulation of viral RNA 2.

Keywords: 2A(HP) protein; ArMV; GFLV; Nepovirus; Phylogeny; RNA 2; Replication; Secoviridae.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Cloning, Molecular
  • Gene Deletion
  • Gene Expression Regulation, Viral / physiology*
  • Molecular Sequence Data
  • Mosaic Viruses / genetics
  • Mosaic Viruses / metabolism*
  • Nepovirus / genetics*
  • Nepovirus / metabolism*
  • Phylogeny
  • RNA, Viral / metabolism*
  • Viral Proteins / genetics
  • Viral Proteins / metabolism*

Substances

  • RNA, Viral
  • Viral Proteins