Neuroprotective effects of 4,5-dimethoxypyrocatechol isolated from Cynanchum paniculatum on HT22 cells

Jin Bae Weon; Bohyoung Lee; Bo-Ra Yun; Jiwoo Lee; Choong Je Ma

doi:10.4103/0973-1296.131028

Neuroprotective effects of 4,5-dimethoxypyrocatechol isolated from Cynanchum paniculatum on HT22 cells

Pharmacogn Mag. 2014 Apr;10(38):161-4. doi: 10.4103/0973-1296.131028.

Authors

Jin Bae Weon¹, Bohyoung Lee¹, Bo-Ra Yun¹, Jiwoo Lee¹, Choong Je Ma²

Affiliations

¹ Department of Medical Biomaterials Engineering, College of Biomedical Science, Kangwon National University, Chuncheon, Korea.
² Department of Medical Biomaterials Engineering, College of Biomedical Science, Kangwon National University, Chuncheon, Korea ; Research Institute of Biotechnology, Kangwon National University, Chuncheon, Korea.

Abstract

Background: Cynanchum paniculatum, belongs to the family Asclepiadaceae and is used to treat various diseases, such as invigorate blood, alleviate edema and to relieve pain and toxicity for a long time.

Materials and methods: 4,5-Dimethoxypyrocatechol was isolated from the 80% methanol extract of C. paniculatum and its neuroprotective effect was evaluated by MTT assay.

Results: 4,5-Dimethoxypyrocatechol had neuroprotective effect on the glutamate-induced cellular oxidative death in HT22 cells.

Conclusion: Furthermore, we found that reactive oxygen species (ROS) accumulation and calcium concentration by oxidative stress were reduced by 4,5-dimethoxypyrocatechol in HT22 cells.

Keywords: 4,5-dimethoxypyrocatechol; Cynanchum paniculatum; HT22 cells; neuroprotective effect.