Complexation of lead by Bermuda grass root exudates in aqueous media

Int J Phytoremediation. 2014;16(6):634-40. doi: 10.1080/15226514.2013.803023.

Abstract

Exudates produced from Bermuda grass roots were collected in deionized water from sterilized Bermuda grass sod at 3-day intervals over a period of 15 days. Exudates were analyzed for total organic carbon, and characterized via Fourier Transform Infrared Spectroscopy. Exudate samples were adjusted to pH values of 4.5, 6.5, and 7.5, amended with lead and quantified for soluble and complexed lead via Inductively Coupled Plasma--Optical Emission Spectrometry. Data obtained from total organic carbon measurements indicated compositional changes in Bermuda grass root exudates as organic carbon concentrations increased over time. Analysis of the infrared spectroscopy data indicated that carboxylic acids and amine functional groups were present in root exudates. Also, the ability of root-exuded compounds to solubilize lead in aqueous media was demonstrated as exudate samples dissolved an average of 60% more lead than deionized water. At pH values 4.5 and 7.5, lead complexation by Bermuda grass root exudates increased with decreasing molecular weight size fractions, while an opposite trend was observed at pH 6.5. Results from this study demonstrated the ability of Bermuda grass root exudates to complex lead in aqueous media.

MeSH terms

  • Biodegradation, Environmental
  • Cynodon / metabolism*
  • Lead / metabolism*
  • Plant Exudates
  • Plant Roots / metabolism
  • Water / chemistry

Substances

  • Plant Exudates
  • Water
  • Lead