The cellulose analysis results of four feedstocks and Avicel obtained by a one-step/two-step hydrolysis method were compared to the conventional cellulose assay according to Updegraff. Slightly lower cellulose levels were observed for Avicel (97%), corn stover (97%), poplar (96%), and Miscanthus (94%) but for pine the amounts were almost identical (101%). Despite these differences, the one-step/two-step method can be seen as a true alternative to the more labor-intensive Updegraff method.
Keywords: Updegraff assay; acid hydrolysis; biomass compositional analysis; cellulose.
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