Isolation of mesenchymal stromal cells from extraembryonic tissues and their characteristics

Bull Exp Biol Med. 2014 May;157(1):119-24. doi: 10.1007/s10517-014-2506-0. Epub 2014 Jun 10.

Abstract

We describe a method of isolation of human mesenchymal stromal cells from the umbilical cord (Wharton's jelly) and human placenta: amnion, placental villi, and trophoblast. Morphology, immunophenotypic characteristics, and differentiation potencies of isolated cells were studied. The capacity of mesenchymal stromal cells from extraembryonic tissues to osteogenic, adipogenic, and chondrogenic differentiation was demonstrated and the dynamics of this process was described. The isolated cells met the criteria for multipotent mesenchymal stem cells.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adipocytes / cytology
  • Adipocytes / immunology
  • Amnion / cytology*
  • Amnion / immunology
  • Antigens, CD / immunology
  • Antigens, CD / metabolism
  • Biomarkers / metabolism
  • Cell Differentiation
  • Cell Separation
  • Cells, Cultured
  • Chondrocytes / cytology
  • Chondrocytes / immunology
  • Chorionic Villi / immunology
  • Female
  • HLA-DR Antigens / immunology
  • HLA-DR Antigens / metabolism
  • Humans
  • Immunophenotyping
  • Mesenchymal Stem Cells / cytology*
  • Mesenchymal Stem Cells / immunology
  • Multipotent Stem Cells / cytology*
  • Multipotent Stem Cells / immunology
  • Osteocytes / cytology
  • Osteocytes / immunology
  • Pregnancy
  • Trophoblasts / cytology
  • Trophoblasts / immunology
  • Umbilical Cord / cytology*
  • Umbilical Cord / immunology
  • Wharton Jelly / cytology*
  • Wharton Jelly / immunology

Substances

  • Antigens, CD
  • Biomarkers
  • HLA-DR Antigens