Objective: To intervene the expression of CD59 on cervical cancer HeLa cells by RNA interference and phage display random peptide library, respectively, and detect the effect of down-regulated CD59 expression on proliferation and apoptosis of the cervical cancer cells.
Methods: HeLa cells were divided into normal control group, CD59 peptide seal group, pSUPER transfected group and pSUPER-siCD59 transfected group. The CD59 peptide seal group was treated with 10 μg/mL CD59 peptide seal for 8 hours. The pSUPER and pSUPER-siCD59 transfected groups were transfected with empty plasmid pSUPER and recombinant plasmid pSUPER-siCD59, respectively. MTT assay was used for the detection of cell proliferation. TUNEL, annexin V-PE/7-AAD staining combined with flow cytometry were adopted for the detection of cell apoptosis.
Results: Compared with the control groups, both CD59 peptide seal group and pSUPER-siCD59 transfected group presented a diminished cell proliferation activity and a strengthened apoptosis. What's more, the effect of peptide seal on CD59 expression inhibition was better than that of pSUPER-siCD59 plasmid.
Conclusion: Down-regulation of CD59 expression could inhibit HeLa cells' proliferation and promote apoptosis, and the inhibitory effect of peptide seal was better than that of CD59 interference plasmid.