Abstract
Interleukin (IL)-17 represents a family of cytokines with six members, namely IL-17A, B, C, D, E, and F. IL-17A and IL-17F are best studied proinflammatory cytokines. CD4(+) T helper cells producing IL-17A have been identified as a distinct T helper subset, Th17 cells. IL-17 and Th17 cells are important mediators in tissue inflammation in immune-mediated inflammatory diseases. IL-17 is also produced by other immune cells and plays an important role in host defense against microbial infection. Cell-based assays are sensitive and quantitative, and enable identification of cellular sources of IL-17 production. This chapter describes usage of flow cytometry and ELISPOT assays to quantify IL-17A-producing cells in disease and in vitro experiments to study T cell function.
Publication types
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Research Support, N.I.H., Extramural
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Antibodies / chemistry
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Brefeldin A / pharmacology
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Enzyme-Linked Immunospot Assay / methods*
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Female
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Flow Cytometry / methods*
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Fluorescein-5-isothiocyanate
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Fluorescent Dyes
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Humans
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Inflammation / immunology
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Inflammation / pathology
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Interleukin-17 / analysis*
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Interleukin-17 / biosynthesis
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Interleukin-17 / immunology
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Interleukin-6 / pharmacology
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Ionomycin / pharmacology
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Mice
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Mice, Inbred C57BL
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Phycoerythrin
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Quinolinium Compounds
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Tetradecanoylphorbol Acetate / pharmacology
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Th17 Cells / cytology
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Th17 Cells / drug effects
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Th17 Cells / immunology*
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Transforming Growth Factor beta / pharmacology
Substances
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Alexa fluor 546
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Antibodies
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Fluorescent Dyes
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IL17A protein, human
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Interleukin-17
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Interleukin-6
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Quinolinium Compounds
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Transforming Growth Factor beta
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Phycoerythrin
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Brefeldin A
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Ionomycin
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Fluorescein-5-isothiocyanate
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Tetradecanoylphorbol Acetate