Antiadherent activity of Schinus terebinthifolius and Croton urucurana extracts on in vitro biofilm formation of Candida albicans and Streptococcus mutans

Dicler S V Barbieri; Fabiana Tonial; Patricia V A Lopez; Beatriz H L N Sales Maia; Germana D Santos; Marina O Ribas; Chirlei Glienke; Vania A Vicente

doi:10.1016/j.archoralbio.2014.05.006

Antiadherent activity of Schinus terebinthifolius and Croton urucurana extracts on in vitro biofilm formation of Candida albicans and Streptococcus mutans

Arch Oral Biol. 2014 Sep;59(9):887-96. doi: 10.1016/j.archoralbio.2014.05.006. Epub 2014 May 13.

Authors

Dicler S V Barbieri¹, Fabiana Tonial², Patricia V A Lopez³, Beatriz H L N Sales Maia⁴, Germana D Santos¹, Marina O Ribas⁵, Chirlei Glienke⁶, Vania A Vicente⁷

Affiliations

¹ Post-Graduation Program Microbiology, Parasitology and Pathology, Basic Pathology Department, Federal University of Parana State, Paraná, Brazil.
² Post-Graduation Program Microbiology, Parasitology and Pathology, Basic Pathology Department, Federal University of Parana State, Paraná, Brazil; Department of Genetics, Federal University of Parana State, Paraná, Brazil.
³ Post-Graduation Program Microbiology, Parasitology and Pathology, Basic Pathology Department, Federal University of Parana State, Paraná, Brazil; Tropical Medicine Institute, Assunção, Paraguay.
⁴ Department of Chemistry, Federal University of Parana State, Paraná, Brazil.
⁵ School of Health and Biological Sciences - Pontifícia Universidade Católica do Paraná, Brazil.
⁶ Department of Genetics, Federal University of Parana State, Paraná, Brazil. Electronic address: cglienke@ufpr.br.
⁷ Post-Graduation Program Microbiology, Parasitology and Pathology, Basic Pathology Department, Federal University of Parana State, Paraná, Brazil; Fellowship from Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq), Brasília, Brazil. Electronic address: vaniava63@gmail.com.

PMID: 24907518
DOI: 10.1016/j.archoralbio.2014.05.006

Abstract

Objective: To evaluate the antiadherent property of crude, methanol and acetate methanol extract fractions from Schinus terebinthifolius and Croton urucurana in hydroalcoholic (HA) and dimethylsulfoxide (DMSO) solvents on in vitro biofilms formed by Streptococcus mutans and Candida albicans strains.

Design: The minimal concentration of adherence (MICA) was determined to evaluate the antiadherent potential of extracts on the in vitro biofilm formation. The extracts of plants were subjected to thin layer chromatography (TLC) in order to detect what class of compounds was responsible for the antiadherent activity. Data were estimated by analysis of variance (ANOVA) complemented by Tukey test level of significance set at 5%.

Results: Both plants demonstrated inhibition of S. mutans and C. albicans on in vitro biofilm formation. The biofilms of C. albicans were more efficiently inhibited by the S. terebinthifolius fraction of acetate-methanol and methanol in hydroalcoholic solvents (p<0.05). The S. mutans biofilms adherence was best inhibited by the S. terebinthifolius crude extract and its methanolic fraction, both in hydroalcoholic solvent (p<0.05). TLC of crude extracts and fractions of S. terebinthifolius detected the presence of several active compounds, including phenolic compounds, anthraquinones, terpenoids, and alkaloids. C. urucurana extracts confirmed activity for both microorganisms (p<0.05). However, higher concentrations were needed to achieve antiadherent activity, mainly to inhibit in vitro biofilm formation of C. albicans.

Conclusion: The antiadherent potential of both plants on in vitro biofilms formed by C. albicans and S. mutans were confirmed, suggesting the importance of studies about these extracts for therapeutic prevention of oral diseases associated with oral biofilms.

Keywords: Adherence; Biofilm; Candida albicans; Plant extracts; Streptococcus mutans.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Anacardiaceae / chemistry*
Anti-Bacterial Agents / pharmacology*
Biofilms / drug effects*
Candida albicans / drug effects*
Cell Adhesion / drug effects*
Chromatography, Thin Layer
Croton / chemistry*
In Vitro Techniques
Microbial Sensitivity Tests
Plant Extracts / pharmacology*
Streptococcus mutans / drug effects*

Substances

Anti-Bacterial Agents
Plant Extracts