Green synthesis of chondroitin sulfate-capped silver nanoparticles: characterization and surface modification

Kuang-ming Cheng; Yao-wen Hung; Cheng-cheung Chen; Cheng-che Liu; Jenn-jong Young

doi:10.1016/j.carbpol.2014.03.053

Green synthesis of chondroitin sulfate-capped silver nanoparticles: characterization and surface modification

Carbohydr Polym. 2014 Sep 22:110:195-202. doi: 10.1016/j.carbpol.2014.03.053. Epub 2014 Mar 28.

Authors

Kuang-ming Cheng¹, Yao-wen Hung¹, Cheng-cheung Chen², Cheng-che Liu¹, Jenn-jong Young³

Affiliations

¹ Institute of Preventive Medicine, National Defense Medical Center, PO Box 90048-700, Sanhsia, New Taipei City 237, Taiwan, ROC.
² Institute of Preventive Medicine, National Defense Medical Center, PO Box 90048-700, Sanhsia, New Taipei City 237, Taiwan, ROC; Department of Food Science, National Taiwan Ocean University, 2 Pei-Ning Road, Keelung 20224, Taiwan, ROC.
³ Institute of Preventive Medicine, National Defense Medical Center, PO Box 90048-700, Sanhsia, New Taipei City 237, Taiwan, ROC. Electronic address: jjyoung@ms49.hinet.net.

PMID: 24906746
DOI: 10.1016/j.carbpol.2014.03.053

Abstract

A one-step route for the green synthesis of highly stable and nanosized silver metal particles with narrow distribution is reported. In this environmentally friendly synthetic method, silver nitrate was used as silver precursor and biocompatible chondroitin sulfate (ChS) was used as both reducing agent and stabilizing agent. The reaction was carried out in a stirring aqueous medium at the room temperature without any assisted by microwave, autoclave, laser irradiation, γ-ray irradiation or UV irradiation. The transparent colorless solution was converted to the characteristics light red then deep red-brown color as the reaction proceeds, indicating the formation of silver nanoparticles (Ag NPs). The Ag NPs were characterized by UV-visible spectroscopy (UV-vis), photon correlation spectroscopy, laser Doppler anemometry, transmission electron microscopy (TEM), and Fourier-transform infrared spectroscopy (FT-IR). The results demonstrated that the obtained metallic nanoparticles were Ag NPs capped with ChS. In this report, dynamic light scattering (DLS) was used as a routinely analytical tool for measuring size and distribution in a liquid environment. The effects of the reaction time, reaction temperature, concentration and the weight ratio of ChS/Ag+ on the particle size and zeta potential were investigated. The TEM image clearly shows the morphology of the well-dispersed ChS-capped Ag NPs are spherical in shape, and the average size (<20 nm) is much smaller than the Z-average value (76.7 nm) measured by DLS. Meanwhile, the ChS-capped Ag NPs coated with N-[(2-hydroxy-3-trimethylammonium) propyl] chitosan chloride (HTCC) were prepared by an ionic gelation method and the surface charge of Ag NPs was switched from negative to positive.

Keywords: Chemical reduction; Chondroitin sulfate; Dynamic light scattering; Green synthesis; N-[(2-hydroxy-3-trimethylammonium)propyl]chitosan chloride; Silver nanoparticles.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Chondroitin Sulfates / chemistry*
Green Chemistry Technology*
Metal Nanoparticles / chemistry*
Metal Nanoparticles / ultrastructure
Particle Size
Silver / chemistry*
Surface Properties

Substances

Silver
Chondroitin Sulfates