Adipokinetic hormone induces changes in the fat body lipid composition of the beetle Zophobas atratus

Marek Gołębiowski; Magdalena Cerkowniak; Aleksandra Urbanek; Małgorzata Słocińska; Grzegorz Rosiński; Piotr Stepnowski

doi:10.1016/j.peptides.2014.05.013

Adipokinetic hormone induces changes in the fat body lipid composition of the beetle Zophobas atratus

Peptides. 2014 Aug:58:65-73. doi: 10.1016/j.peptides.2014.05.013. Epub 2014 Jun 4.

Authors

Marek Gołębiowski¹, Magdalena Cerkowniak², Aleksandra Urbanek³, Małgorzata Słocińska⁴, Grzegorz Rosiński⁴, Piotr Stepnowski²

Affiliations

¹ Laboratory of Natural Products Analysis, Department of Environmental Analysis, Institute for Environmental and Human Health Protection, Faculty of Chemistry, University of Gdansk, ul. Wita Stwosza 63, 80-308, Poland. Electronic address: marek.golebiowski@ug.edu.pl.
² Laboratory of Natural Products Analysis, Department of Environmental Analysis, Institute for Environmental and Human Health Protection, Faculty of Chemistry, University of Gdansk, ul. Wita Stwosza 63, 80-308, Poland.
³ Department of Invertebrate Zoology and Parasitology, University of Gdańsk, Wita Stwosza 59, 80-308 Gdańsk, Poland.
⁴ Department of Animal Physiology and Development, Adam Mickiewicz University, Umultowska 89, 61-614 Poznan, Poland.

PMID: 24905623
DOI: 10.1016/j.peptides.2014.05.013

Abstract

In insects, neuropeptide adipokinetic hormone (AKH) released from the corpora cardiaca mobilizes lipids and carbohydrates in the fat body. We examined the developmental differences in the action of Tenmo-AKH, a bioanalogue belonging to the adipokinetic/hypertrahelosemic family (AKH/HrTH), on the lipid composition of larval and pupal fat bodies in the beetle Zophobas atratus. Tenmo-AKH was administered to the beetle larvae and pupae either as a single dose or as two doses of 20 pmol during a 24h interval. Extracts of fat bodies were used to analyse the lipid composition by gas chromatography (GC) combined with mass spectrometry (GC-MS). Control extracts were analyzed using the same method. Fatty acids (FA) and fatty acid methyl esters (FAME) were the most abundant compounds in the fat bodies from both developmental stages. We observed significant differences in their concentrations following hormonal treatment. Tenmo-AKH also induced a distinct increase in larval sterols, fatty alcohols and benzoic acid.

Keywords: Adipokinetic hormone; Fat body; GC–MS; Lipids; Zophobas atratus.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Adipokines / pharmacology*
Animals
Coleoptera / metabolism*
Fat Body / metabolism*
Insect Proteins / pharmacology*
Lipid Metabolism / drug effects*

Substances

Adipokines
Insect Proteins