Abstract
The key step in high quality microbial matrix-assisted laser desorption/ionization mass spectrometry imaging (microbial MALDI MSI) is the fabrication of a homogeneous matrix coating showing a fine-grained morphology. This application note addresses a novel method to apply solid MALDI matrices onto microbial cultures grown on thin agar media. A suspension of a mixture of 2,5-DHB and α-CHCA is sprayed onto the agar sample surface to form highly homogeneous matrix coatings. As a result, the signal intensities of metabolites secreted by the fungus Aspergillus fumigatus were found to be clearly enhanced.
Publication types
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Comparative Study
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Research Support, Non-U.S. Gov't
MeSH terms
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Acetates / chemistry
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Aspergillus fumigatus / growth & development
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Aspergillus fumigatus / metabolism*
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Chloroform / chemistry
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Coumaric Acids / chemistry*
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Gentisates / chemistry*
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Indicators and Reagents / chemistry*
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Methylene Chloride / chemistry
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Microscopy, Fluorescence
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Molecular Imaging / methods*
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Particle Size
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Propionates
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Solvents / chemistry
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Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
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Tandem Mass Spectrometry
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Volatilization
Substances
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Acetates
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Coumaric Acids
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Gentisates
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Indicators and Reagents
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Propionates
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Solvents
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Methylene Chloride
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Chloroform
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p-coumaric acid
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2,5-dihydroxybenzoic acid
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methyl acetate