Second generation sequencing of three STRs D3S1358, D12S391 and D21S11 in Danes and a new nomenclature for sequenced STR alleles

Chiara Gelardi; Eszter Rockenbauer; Sigrun Dalsgaard; Claus Børsting; Niels Morling

doi:10.1016/j.fsigen.2014.04.016

Second generation sequencing of three STRs D3S1358, D12S391 and D21S11 in Danes and a new nomenclature for sequenced STR alleles

Forensic Sci Int Genet. 2014 Sep:12:38-41. doi: 10.1016/j.fsigen.2014.04.016. Epub 2014 May 10.

Authors

Chiara Gelardi¹, Eszter Rockenbauer², Sigrun Dalsgaard³, Claus Børsting³, Niels Morling³

Affiliations

¹ Section of Forensic Genetics, Department of Forensic Medicine, Faculty of Health and Medical Sciences, University of Copenhagen, Denmark; Faculty of Mathematical, Physical and Natural Sciences, University of Palermo, Palermo, Italy.
² Section of Forensic Genetics, Department of Forensic Medicine, Faculty of Health and Medical Sciences, University of Copenhagen, Denmark. Electronic address: eszter.rockenbauer@sund.ku.dk.
³ Section of Forensic Genetics, Department of Forensic Medicine, Faculty of Health and Medical Sciences, University of Copenhagen, Denmark.

PMID: 24893347
DOI: 10.1016/j.fsigen.2014.04.016

Abstract

Second generation sequencing (SGS) may revolutionize the field of forensic STR typing. Two of the essential requirements for implementation of an SGS based approach for forensic investigations are (1) establishment of adequate frequency databases and (2) adoption of a new STR nomenclature. We report the STR sequences and allele frequencies of three STR loci: D3S1358, D12S391 and D21S11 in 197 unrelated Danes. We used a new STR nomenclature that depicts the locus name used in forensic genetics, the length of the repeat region divided by the repeat length (typically 4 nucleotides) and detailed sequence information of possible sub-repeats and SNPs within the amplified fragment.

Keywords: Forensic genetics; Frequency database; Nomenclature; STR; Second generation sequencing.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Denmark
Gene Frequency*
Humans
Microsatellite Repeats*
Sequence Analysis, DNA / methods*
Terminology as Topic*