Parvovirus B19 VLP recognizes globoside in supported lipid bilayers

Virology. 2014 May:456-457:364-9. doi: 10.1016/j.virol.2014.04.004. Epub 2014 Apr 28.

Abstract

Studies have suggested that the glycosphingolipid globoside (Gb4Cer) is a receptor for human parvovirus B19. Virus-like particles bind to Gb4Cer on thin-layer chromatograms, but a direct interaction between the virus and lipid membrane-associated Gb4Cer has been debated. Here, we characterized the binding of parvovirus B19 VP1/VP2 virus-like particles to glycosphingolipids (i) on thin-layer chromatograms (TLCs) and (ii) incorporated into supported lipid bilayers (SLBs) acting as cell-membrane mimics. The binding specificities of parvovirus B19 determined in the two systems were in good agreement; the VLP recognized both Gb4Cer and the Forssman glycosphingolipid on TLCs and in SLBs compatible with the role of Gb4Cer as a receptor for this virus.

Keywords: Carbohydrate; Glycan; Glycobiology; Glycosphingolipids; Parvovirus B19 virus-like particles.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Capsid Proteins / metabolism
  • Globosides / metabolism*
  • Lipid Bilayers / metabolism*
  • Parvovirus B19, Human / physiology*
  • Receptors, Virus / metabolism*
  • Virosomes / metabolism
  • Virus Attachment*

Substances

  • Capsid Proteins
  • Globosides
  • Lipid Bilayers
  • Receptors, Virus
  • Virosomes
  • capsid protein VP1, parvovirus B19
  • capsid protein VP2, parvovirus B19