Abstract
This work describes the formation of a new dendritic polyglycerol-poly(ethylene glycol)-based 3D polymer network as a matrix for immobilization of the redox enzyme periplasmatic aldehyde oxidoreductase to create an electrochemical biosensor. The novel network is built directly on the gold surface, where it simultaneously stabilizes the enzyme for up to 4 days. The prepared biosensors can be used for amperometric detection of benzaldehyde in the range of 0.8-400 μM.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Benzaldehydes / chemistry
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Benzaldehydes / isolation & purification*
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Biosensing Techniques / methods*
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Dendrimers / chemistry*
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Enzymes, Immobilized / chemistry*
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Glucose Oxidase / chemistry
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Glycerol / chemistry
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Oxidation-Reduction
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Polyethylene Glycols / chemistry
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Polymers / chemistry
Substances
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Benzaldehydes
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Dendrimers
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Enzymes, Immobilized
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Polymers
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polyglycerol
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Polyethylene Glycols
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Glucose Oxidase
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Glycerol
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benzaldehyde