Generation of an infectious Negev virus cDNA clone

J Gen Virol. 2014 Sep;95(Pt 9):2071-2074. doi: 10.1099/vir.0.066019-0. Epub 2014 May 30.

Abstract

The genus Negevirus consists of insect-only viruses isolated from mosquitoes and sandflies. Here, we report the successful construction of a full-length infectious cDNA clone of Negev virus (NEGV) strain M30957. Viral RNA was transcribed in vitro and virus was readily rescued with or without the use of a cap analogue. These results strongly suggest that NEGV, and likely other members within the genus, is a non-segmented, single-stranded, positive-sense RNA virus.

Publication types

  • Research Support, N.I.H., Extramural

MeSH terms

  • Animals
  • Cells, Cultured
  • Cloning, Molecular
  • DNA, Complementary / genetics*
  • Genome, Viral
  • Insect Viruses / genetics*
  • Insecta / virology*
  • RNA Viruses / classification*
  • RNA Viruses / genetics*
  • RNA, Viral / genetics

Substances

  • DNA, Complementary
  • RNA, Viral