A "light-up" and "spectrum-shift" response of aptamer-functionalized silver nanoclusters for intracellular mRNA imaging

Chem Commun (Camb). 2014 Jul 11;50(54):7107-10. doi: 10.1039/c4cc00160e.

Abstract

We have designed a novel multifunctional DNA scaffold for the synthesis of fluorescent silver nanoclusters (Ag NCs) using a one-pot approach. The obtained DNA/Ag NCs presented a "light-up" and "spectrum-shift" response to target DNA in vitro and could further image the tumor-related mRNA in living cells.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Aptamers, Nucleotide / chemistry*
  • Cell Survival / drug effects
  • Diagnostic Imaging
  • Fluorescent Dyes / chemistry*
  • HeLa Cells
  • Humans
  • Mice
  • NIH 3T3 Cells
  • Nanostructures / chemistry*
  • RNA, Messenger / analysis*
  • Silver / chemistry*

Substances

  • Aptamers, Nucleotide
  • Fluorescent Dyes
  • RNA, Messenger
  • Silver