Facile synthesis of PSMA-g-3ABA/MWCNTs nanocomposite as a substrate for hemoglobin immobilization: application to catalysis of H(2)O(2)

Mehdi Baghayeri; Ehsan Nazarzadeh Zare; Reza Hasanzadeh

doi:10.1016/j.msec.2014.03.012

Facile synthesis of PSMA-g-3ABA/MWCNTs nanocomposite as a substrate for hemoglobin immobilization: application to catalysis of H(2)O(2)

Mater Sci Eng C Mater Biol Appl. 2014 Jun 1:39:213-20. doi: 10.1016/j.msec.2014.03.012. Epub 2014 Mar 12.

Authors

Mehdi Baghayeri¹, Ehsan Nazarzadeh Zare², Reza Hasanzadeh³

Affiliations

¹ Department of Chemistry, Faculty of Science, Hakim Sabzevari University, P.O. Box 397, Sabzevar, Iran. Electronic address: m.baghayeri@hsu.ac.ir.
² Department of Polymer Chemistry, Faculty of Chemistry, University of Mazandaran, Babolsar, Iran.
³ Department of Chemistry, Faculty of Science, Urmia University, Urmia, Iran.

PMID: 24863218
DOI: 10.1016/j.msec.2014.03.012

Abstract

The new nanocomposite films based on poly(styrene-alternative-maleic anhydride) grafted to 3-aminobenzoic acid (PSMA-g-3ABA) and multi-walled carbon nanotubes (MWCNTs) were applied to immobilize hemoglobin (Hb) for biosensor fabrication (PSMA-g-3ABA/MWCNTs). Electrochemical impedance spectroscopy was used to confirm the adsorption of Hb onto the surface of PSMA-g-3ABA/MWCNTs. The immobilized Hb maintains its bioactivities and displays an excellent electrochemical behavior. The biosensor was used to catalyze the reduction of hydrogen peroxide. The electrocatalytic response showed a linear dependence on the H2O2 concentration ranging widely from 1.0×10(-6)M to 5.0×10(-4)M with a detection limit of 3.2×10(-7)M. The apparent Michaelis-Menten constant of Hb on the modified electrode was estimated to be 0.22mM. The proposed method opens a way to develop biosensors by using nanostructured materials with low electrical conductivity.

Keywords: Biosensor; Electrocatalysis; Hemoglobin; Nanocomposite; Poly(styrene-alternative-maleic anhydride).

MeSH terms

Biocompatible Materials / chemistry
Biosensing Techniques / instrumentation
Biosensing Techniques / methods
Catalysis
Electrochemistry / methods
Electrodes
Hemoglobins / chemistry*
Hydrogen Peroxide / chemistry
Immobilized Proteins / chemistry*
Limit of Detection
Maleates / chemical synthesis*
Nanocomposites / chemistry*
Nanotubes, Carbon / chemistry*
Polystyrenes / chemical synthesis*
meta-Aminobenzoates / chemical synthesis*

Substances

Biocompatible Materials
Hemoglobins
Immobilized Proteins
Maleates
Nanotubes, Carbon
Polystyrenes
meta-Aminobenzoates
poly(styrene-alt-maleic anhydride)
Hydrogen Peroxide
3-aminobenzoic acid