Analysis of etoxazole in red pepper after major modification of QuEChERS for gas chromatography-nitrogen phosphorus detection

Biomed Chromatogr. 2014 Jun;28(6):767-73. doi: 10.1002/bmc.3130.

Abstract

A major modification to the QuEChERS (quick, easy, cheap, effective, rugged and safe) method was developed for the analysis of etoxazole in red pepper using gas chromatography coupled with a nitrogen-phosphorus detector. Etoxazole was extracted with acetonitrile, partitioned with magnesium sulfate and purified with a solid-phase extraction cartridge. The method showed good linearity with a determination coefficient (R(2) ) of 0.998 for the 0.02-2.0 mg/L concentration range. The method was validated using blank red pepper spiked at 0.2 and 1.0 mg/kg, and the average recovery rate was 74.4-79.1% with relative standard deviations <5% for intra- and inter-day precision. The limits of detection and quantification were 0.007 and 0.02 mg/kg, respectively. The developed method was successfully applied to field-incurred samples, and the presence of etoxazole residues was confirmed using gas chromatography/mass spectrometry.

Keywords: Etoxazole; QuEChERS; gas chromatography; red pepper; residue analysis.

Publication types

  • Evaluation Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Capsicum / chemistry*
  • Chromatography, Gas / instrumentation
  • Chromatography, Gas / methods
  • Food Contamination / analysis
  • Oxazoles / analysis*
  • Oxazoles / isolation & purification*
  • Pesticide Residues / analysis*
  • Pesticide Residues / isolation & purification*
  • Solid Phase Extraction / methods*

Substances

  • Oxazoles
  • Pesticide Residues
  • etoxazole