Lycium seedling was subjected to varying doses of V for 40-45 days to examine the effects on uptake, accumulation, and speciation of V in Lycium seedling by differential centrifugation and enzymolysis. V concentrations in Lycium seedling organs were in sequence as follows: root>leaf>stem. V uptake into stem and leaf were primarily combined with acid-soluble polar compounds, polysaccharide, and immobile materials on the cell walls. There were different speciations of V in root with different V stress levels. Enzymolysis results suggest that about 60% of the V in Lycium seedling root was combined with pectin and cellulose. It is the antidotal effect of pectin and cellulose in the cell wall that reduced the V damage to Lycium seedling.