Co-expression of EtMic2 protein and chicken interleukin-18 for DNA vaccine against chicken coccidiosis

Wenyan Shi; Qing Liu; Jie Zhang; Jingjing Sun; Xiyue Jiang; Jing Geng; Fangkun Wang; Yihong Xiao; Hongmei Li; Xiaomin Zhao

doi:10.1016/j.rvsc.2014.05.001

Co-expression of EtMic2 protein and chicken interleukin-18 for DNA vaccine against chicken coccidiosis

Res Vet Sci. 2014 Aug;97(1):64-70. doi: 10.1016/j.rvsc.2014.05.001. Epub 2014 May 14.

Authors

Wenyan Shi¹, Qing Liu¹, Jie Zhang¹, Jingjing Sun¹, Xiyue Jiang¹, Jing Geng¹, Fangkun Wang¹, Yihong Xiao², Hongmei Li³, Xiaomin Zhao⁴

Affiliations

¹ Department of Preventive Veterinary Medicine, College of Veterinary Medicine, Shandong Agricultural University, 61 Daizong Street, Taian City, Shandong Province 271018, China; Shandong Provincial Key Laboratory of Animal Biotechnology and Disease Control and Prevention, Shandong Agricultural University, 61 Daizong Street, Taian City, Shandong Province 271018, China.
² Department of Basic Veterinary Medicine, College of Veterinary Medicine, Shandong Agricultural University, 61 Daizong Street, Taian City, Shandong Province 271018, China.
³ Department of Preventive Veterinary Medicine, College of Veterinary Medicine, Shandong Agricultural University, 61 Daizong Street, Taian City, Shandong Province 271018, China; Shandong Provincial Key Laboratory of Animal Biotechnology and Disease Control and Prevention, Shandong Agricultural University, 61 Daizong Street, Taian City, Shandong Province 271018, China. Electronic address: lihm@sdau.edu.cn.
⁴ Department of Preventive Veterinary Medicine, College of Veterinary Medicine, Shandong Agricultural University, 61 Daizong Street, Taian City, Shandong Province 271018, China; Shandong Provincial Key Laboratory of Animal Biotechnology and Disease Control and Prevention, Shandong Agricultural University, 61 Daizong Street, Taian City, Shandong Province 271018, China. Electronic address: xmzhao66@163.com.

PMID: 24856455
DOI: 10.1016/j.rvsc.2014.05.001

Abstract

In the present study, a naked EtMIC2 DNA vaccine, a ChIL-18 expression vector and a EtMIC2 and ChIL-18 co-expression DNA vaccine were constructed and their protective efficacies against homologous challenge were compared and evaluated by examining the body weight gain, oocyst shedding, cecal lesion, ACI as well as specific anti-EtMic2 antibody level, the proliferation ability and percentages of CD4+ and CD8+ of splenocytes. The results showed the naked EtMIC2 DNA vaccine could increase the weight gain and decrease the oocyst shedding, but could not alleviate the cecal lesion of immunized chickens compared to unimmunized chickens. Chickens immunized with the co-expression vector pVAX1-MIC2-IL-18 exhibited much improved immune protection against challenge compared to chickens immunized with naked EtMIC2 DNA vaccine, or with naked EtMIC2 DNA vaccine and ChIL-18 expression vector applied separately. These results suggest that the co-expression of ChIL-18 with EtMic2 together could significantly improve the immune protection of the EtMic2 protein.

Keywords: ChIL-18; Co-expression; DNA vaccine; EtMIC2.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Blotting, Western
CD4-CD8 Ratio
Cecum / pathology
Chickens
Coccidiosis / prevention & control
Coccidiosis / veterinary*
DNA Primers / genetics
Escherichia coli
Gene Expression Profiling
Interleukin-18 / immunology*
Microscopy, Fluorescence
Oocysts / metabolism
Plasmids / genetics
Poultry Diseases / parasitology*
Poultry Diseases / prevention & control*
Protozoan Vaccines / genetics
Protozoan Vaccines / immunology*
Vaccination / veterinary
Vaccines, DNA / genetics
Vaccines, DNA / immunology*
Weight Gain

Substances

DNA Primers
Interleukin-18
Protozoan Vaccines
Vaccines, DNA