Cryo-scanning electron microscopy to study the freezing behavior of plant tissues

Seizo Fujikawa; Keita Endoh

doi:10.1007/978-1-4939-0844-8_10

Cryo-scanning electron microscopy to study the freezing behavior of plant tissues

Methods Mol Biol. 2014:1166:99-116. doi: 10.1007/978-1-4939-0844-8_10.

Authors

Seizo Fujikawa¹, Keita Endoh

Affiliation

¹ Faculty of Agriculture, Hokkaido University, Kita 9, Nishi 9, Kita-ku, Sapporo, 060-8589, Japan, sfuji@for.agr.hokudai.ac.jp.

PMID: 24852632
DOI: 10.1007/978-1-4939-0844-8_10

Abstract

A cryo-scanning electron microscope (cryo-SEM) is a valuable tool for observing bulk frozen samples to monitor freezing responses of plant tissues and cells. Here, essential processes of a cryo-SEM to observe freezing behaviors of plant tissue cells are described.

MeSH terms

Cell Wall / chemistry
Cryoelectron Microscopy / methods*
Extracellular Space / chemistry
Freezing*
Ice
Microscopy, Electron, Scanning / methods*
Plants / chemistry*
Plants / ultrastructure*
Tissue Fixation

Substances

Ice