Synthetic disialyl hexasaccharides protect neonatal rats from necrotizing enterocolitis

Angew Chem Int Ed Engl. 2014 Jun 23;53(26):6687-91. doi: 10.1002/anie.201403588. Epub 2014 May 21.

Abstract

Two novel synthetic α2-6-linked disialyl hexasaccharides, disialyllacto-N-neotetraose (DSLNnT) and α2-6-linked disialyllacto-N-tetraose (DS'LNT), were readily obtained by highly efficient one-pot multienzyme (OPME) reactions. The sequential OPME systems described herein allowed the use of an inexpensive disaccharide and simple monosaccharides to synthesize the desired complex oligosaccharides with high efficiency and selectivity. DSLNnT and DS'LNT were shown to protect neonatal rats from necrotizing enterocolitis (NEC) and are good therapeutic candidates for preclinical experiments and clinical application in treating NEC in preterm infants.

Keywords: disialyl glycans; enzymatic synthesis; enzymes; necrotizing enterocolitis; oligosaccharides.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Animals
  • Bifidobacterium / enzymology
  • Drug Evaluation, Preclinical
  • Enterocolitis, Necrotizing / drug therapy*
  • Multienzyme Complexes / metabolism
  • Oligosaccharides / chemical synthesis*
  • Oligosaccharides / chemistry
  • Oligosaccharides / therapeutic use
  • Protective Agents / chemical synthesis
  • Protective Agents / chemistry
  • Protective Agents / therapeutic use*
  • Rats

Substances

  • Multienzyme Complexes
  • Oligosaccharides
  • Protective Agents
  • disialyllacto-N-tetraose