Abstract
Previously, we reported that MALDI spectra of peptides became reproducible when temperature was kept constant. Linear calibration curves derived from such spectral data could be used for quantification. Homogeneity of samples was one of the requirements. Among the three popular matrices used in peptide MALDI [i.e., α-cyano-4-hydroxycinnamic acid (CHCA), 2,5-dihydroxybenzoic acid (DHB), and sinapinic acid (SA)], homogeneous samples could be prepared by conventional means only for CHCA. In this work, we showed that sample preparation by micro-spotting improved the homogeneity for all three cases.
Publication types
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Comparative Study
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Research Support, Non-U.S. Gov't
MeSH terms
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Analytic Sample Preparation Methods*
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Calibration
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Coumaric Acids / chemistry
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Electrochemical Techniques
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Gentisates / chemistry
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Limit of Detection
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Membranes, Artificial
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Microchemistry / methods
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Molecular Weight
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Oligopeptides / analysis*
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Oligopeptides / chemistry
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Reproducibility of Results
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Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
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Surface Properties
Substances
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Coumaric Acids
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Gentisates
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Membranes, Artificial
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Oligopeptides
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alpha-cyano-4-hydroxycinnamate
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sinapinic acid
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2,5-dihydroxybenzoic acid