Purification and identification of antioxidant peptides from peanut protein isolate hydrolysates using UHR-Q-TOF mass spectrometer

Na Ji; Cuixia Sun; Yunxia Zhao; Liu Xiong; Qingjie Sun

doi:10.1016/j.foodchem.2014.04.010

Purification and identification of antioxidant peptides from peanut protein isolate hydrolysates using UHR-Q-TOF mass spectrometer

Food Chem. 2014 Oct 15:161:148-54. doi: 10.1016/j.foodchem.2014.04.010. Epub 2014 Apr 12.

Authors

Na Ji¹, Cuixia Sun¹, Yunxia Zhao¹, Liu Xiong¹, Qingjie Sun²

Affiliations

¹ School of Food Science and Engineering, Qingdao Agricultural University, Qingdao, Shandong Province 266109, China.
² School of Food Science and Engineering, Qingdao Agricultural University, Qingdao, Shandong Province 266109, China. Electronic address: phdsun@163.com.

PMID: 24837933
DOI: 10.1016/j.foodchem.2014.04.010

Abstract

Peanut protein isolate (PPI) was hydrolysed with alcalase to obtain antioxidant peptides. To purify these peptides, the peanut protein isolate hydrolysates (PPIH) were separated by ultrafiltration (MWCO=3kDa) and the obtained PPIH-II (Mw<3kDa) with higher antioxidant activity was further separated by gel filtration chromatography (Sephadex G-15). After filtration, both peptides, P1 and P4, with stronger antioxidant capacity were fractionated using preparative high performance liquid chromatography (P-HPLC). Three antioxidant peptides were finally purified from P1 and P4 using the UHR-Q-TOF mass spectrometer, and the amino acid sequences of the peptides were identified as Thr-Pro-Ala (286kDa), Ile/Leu-Pro-Ser (315kDa) and Ser-Pro (202kDa), respectively.

Keywords: Amino acid sequence; Antioxidant peptides; Identification; Peanut protein isolate hydrolysates.

MeSH terms

Amino Acid Sequence
Antioxidants / chemistry*
Arachis / chemistry*
Mass Spectrometry / methods*
Molecular Sequence Data
Peptides / chemistry*
Protein Hydrolysates

Substances

Antioxidants
Peptides
Protein Hydrolysates